Original articles
Local reference ranges for full blood count and CD4 lymphocyte count testing
Abstract
Methods. A cross-sectional study of 631 female and 88 male HIV negative participants from the Gauteng region was performed. Full blood count, automated differential and CD4 count analyses were done using the latest internationally accepted technology. Reference ranges were compiled from the 2.5th and 97.5th percentiles for both male and female participant groups and gender and ethnic comparisons calculated by non-parametric tests.
Results: Results of 41 females were removed from statistical analysis as their results were suggestive of possible anaemia. Full blood count reference interval comparison confirmed gender specific differences in red blood cell and platelet parameters. Ethnic specific differences were found for some red blood cell parameters in the Black female cohort. In addition, both Black males and females generally had lower neutrophil and higher lymphocyte counts than a combined Asian/Caucasian/Coloured ethnic group.
Conclusion. Comparison of the currently calculated reference ranges to published data and reference values in use indicated that a separate ethnic specific reference range be introduced for the percentage/absolute neutrophil count and percentage lymphocytes. In addition, locally derived reference ranges for RDW and CD4 percentage of lymphocytes should be implemented for routine diagnostic testing.
Authors' affiliations
Denise Lawrie, NHLS
Lindi Marie Coetzee, NHLS
Piet Becker, MRC
Johnny Mahlangu, NHLS
Wendy Stevens, NHLS
Deborah Kim Glencross, NHLS
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Date published: 2009-04-03
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